Set of Bioinks Allows Complex High-Resolution Bio-Structures from Micro- to Meso-Scale

Cooperation of UpNano and BIO INX yields further advancement: Two-stage 3D-printing of organ-on-chip devices with encapsulated live cells is possible.

Vienna, Austria, 12 June, 2023 – The launch of a new biocompatible hydrogel resin marks the begin of a new era in bioprinting. The novel resin allows 2-photon polymerization (2PP) 3D-printing from micro- to meso-scale at highest resolution. The reactivity and easy processing of the material permits the fabrication of complex and versatile organ- or lab-on-chip designs. This new bioink called Hydrotech INX© U200 has been developed by the material specialist BIO INX (Belgium) in collaboration with UpNano (Austria), leading manufacturer of 2PP 3D-printers. The results of this collaboration now allow to add an additional layer of complexity to a bioprinted construct by the smart combination of multiple biocompatible materials. In this respect versatile cancer-on-chip models are possible by combining the newly launched resin with cell-encapsulating gelatin-based hydrogel Hydrobio INX© U200, which is the first commercially available high resolution bio ink that offers cell interactivity.

The ever-increasing possibilities of bioprinting are nurtured by a continuous advancement of 3D-bioprinting technologies. In this, 2-photon polymerization (2PP) 3D-printing excels as it covers the entire spectrum of biological dimensions with one printing technology: sub-micrometer resolution combined with the capability of printing objects at centimeter dimensions (macro-scale). This impressive potential has now been advanced by a set of bioinks jointly developed by BIO INX, 3D-bioprinting material specialist, and UpNano, leading manufacturer of 2PP 3D-printers.

Macrostructures with Micrometer-Precision

With the release of Hydrotech INX© U200 the two companies demonstrate their success in developing a versatile material that enables 2PP 3D-printing of macrostructures with micrometer-precision. The material has been certified according to ISO 10993-5, confirming its excellent biocompatibility. Jasper Van Hoorick, CEO of BIO INX, comments: “In combination with the fact that the material is completely bio-inert, its robust characteristics make Hydrotech INX© U200 the ideal choice for organ-on-chip applications.”

However, in order to benefit from the full potential of the novel bioink, a state-of-the-art high-resolution 3D printer is required. Here, UpNano’s NanoOne 2PP-printer range offers a very suitable choice. The NanoOne is the fastest high-resolution 3D printing system on the market. It is based on multiphoton lithography and combines the precision of 2PP with an unmatched throughput of up to 450 mm³ per hour. On top of that, the NanoOne Bio is specifically adapted for printing with living cells and under sterile conditions – and thus a congenial partner for the most challenging task in bioprinting: fabrication of complex 3D scaffolds for cell cultures whilst embedding living cells into these structures in one simple set-up.

Complex Design – Simple Production

“Combining these steps can easily be done,” explains Denise Hirner, COO and co-founder of UpNano, “by using the NanoOne Bio in combination with the two bioinks Hydrotech INX© U200 for the inert structure and Hydrobio INX© U200 for living cells in 3 dimensions.” In fact, whilst the Hydrotech-bioink is perfectly optimized for the high-resolution fabrication of bioinert but biocompatible microstructures, the Hydrobio-bioink is a water-soluble natural hydrogel that allows the direct transfer of cell cultures from 2D-culture plates into complex 3D-structures. It has been specifically developed for the encapsulation of multiple cell types thereby allowing the generation of complex 3D-microtissues.

Production of lab-on-a-chip devices will now be possible not only with an unprecedented precision, but also with directly embedded living cells – thereby saving time and improving the significance of the results. Surface structures resembling natural tissues (biomimetic structures) such as liver lobules or intestinal villi can now be created inside a microfluidic channel on a chip, and living cells at various densities can subsequently be seeded directly onto them.

By combining their respective competences in material development and 2PP 3D-printing, BIO INX and UpNano are pushing the possibilities of bioprinting to a new level and will continue doing so.

About UpNano GmbH

UpNano is a Vienna (Austria) based high-tech company with the focus on development, manufacturing, and commercialization of high-resolution 3D-printers. The systems are based on 2-photon-polymerization which offer industry-leading speed and resolution below 0.2 µm. UpNano is committed to providing customers with a holistic package of hardware, software, and optimized printing materials, for the fabrication of polymeric microparts as well as the unique possibility of bioprinting in a native cell environment. Using UpNano’s cutting-edge technology makes it possible to print objects with sizes ranging from the sub-micrometer to the centimeter range and up to 40 mm in height – within times and precision never achieved before.

About BIO INX

BIO INX is a start-up focusing on the development and commercialization of biomaterials for 3D-bioprinting applications. It is leading manufacturer of high-resolution resins for bioprinting. The company is committed to providing innovative solutions that enable researchers and clinicians to create advanced biomedical structures. BIO INX offers a range of products that are optimized for various bioprinting techniques, including extrusion, inkjet, DLP and two-photon polymerization. By offering a unique material portfolio with a diverse range of material properties, the applications become nearly limitless: the bioinks can be applied for various tissue types with applications in regenerative medicine and drug and cosmetics screening.

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Lattice structure made of Hydrotech INX© U200 printed with a NanoOne printing system. Component dimensions 3.25x3.25x20 mm3 printed in proprietary vat mode by UpNano.

© UpNano GmbH
Multimaterial microfludic chip printed under sterile conditions using the NanoOne printing system. Outer geometry made from Hydrotech INX© U200, inner scaffold structures printed from cell compatible Hydrobio INX© U200 and seeded with cells.

© BIO INX
Hydrotech INX© U200 material kit developed by the material specialist BIO INX.

© BIO INX
Hydrobio INX© U200 material kit developed by the material specialist BIO INX.

© BIO INX

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