The new dimension in 3D bioprinting

NanoOne Bio allows 3D printing of living cells for biological applications with unprecedented precision. Cell models mimicking natural tissue topography and sterile fabrication of cell scaffolds herald a new era for biomedical research.

Photo: NanoOne Bio
Photo: a drop of resin is being placed into a petri disch with a pipette

Biocompatible 3D printing in the presence of living cells

Hydrobio INX© U200 is a gelatin-based hydrogel developed specifically for UpNano by BioINX. It provides all the biological benefits of conventional gelatin-based materials in combination with two photon polymerization processability enhanced by a high reactivity and fast curing speed. As a result, the bioink can be processed in the presence of cells at fast writing speeds resulting in a high cell viability. It is based on gelatin type B derived from natural collagen, which is modified with photo processable functional groups. After processing, it mimics the natural extra cellular matrix to a large extent resulting in a high cell viability. Additionally, as the formed hydrogel is biodegradable, it allows cells to remodel the environment and substitute it with newly formed ECM over time.

ASC: adipose tissue

HUVEC: blood vessels

Osteoblasts: bone tissue

Corneal endothelial cells: cornea

Stage-top incubation system

The UpNano Bio unit is the centrepiece of the bio variant of the innovative NanoOne printing system. The incubation system with its specifically designed insert can be simply clicked into the building platform. Necessary tubings and cables can be accessed by an interchangeable opening in the door. The incubation system’s temperature control unit makes it possible to adjust humidity and CO2 content to meet the requirements of the cell type used, ensuring a native environment during the print process and thus cell viability. The biounit is based on the ibidi stage top incubation system universal fit and adapted and integrated into the NanoOne Bio system

The incubation system is based on the ibidi Stage Top Incubation System, Universal Fit, and adapted and integrated into the NanoOne Bio system.

Incubation system stage insert for temperature CO2 and humidity control during the printing process

Bio unit: stage top incubation system control unit of the NanoOne Bio printing system

8-well chamber

96-well plate

Petri dish

Infographic: High-resolution bioprinting

High-resolution bioprinting

The NanoOne printing system enables direct printing of living cells

Cells embedded in an X Hydrobio INX matrix can be used for three-dimensional in-vitro cell tests, which have become increasingly important in cell culture, tissue regeneration and pharmaceutical research. There is hardly any restriction in the choice of substrate. Cell culture plates, Petri dishes or microfluidic chips with glass bottoms can be inserted into the machine with the help of the universal holder. In combination with the stage top incubation system, native temperature, humidity and CO2 conditions are controlled during the printing process, ensuring call viability.

Biocompatible structures

Fabrication of structures and surface textures mimicking the microenvironment of cells

UpNano’s materials allow the fabrication of structures and surface textures mimicking the microenvironment of cells. These three-dimensional culture approaches, and especially the results obtained from them, are becoming increasingly important in preclinical research and will be groundbreaking for future therapeutic strategies.

The non-cytotoxicity and high biocompatibility of UpPhoto and UpOpto have been certified according to EN ISO 10992-5:2009. In addition, the UpOpto material has a low fluorescence, which is very advantageous for cell biological applications.

Infographic: Biocompatible structures
Infographic: microfluidic applications

Microfluidic applications

Fabrication of high-resolution structures directly within a microfluidic chip

Internal elements such as separators, channels or membranes can be fabricated directly within a commercially available or custom-made microfluidic chip. The printing process takes place under sterile conditions. The material is prepared and injected under the laminar hood and the sealed chip can then be placed in the printing system and postprocessed in a sterile environment.

When using the biocompatible and low fluorescent UpOpto printing material, interference with any fluorescent signals of the cells can be avoided.